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Shon et al. ([2008]) reported that CAT activity had exhibited a decreasing tendency upon submerging stress in young rice seedlings, and appeared as the most sensitive enzyme to stress among antioxidant components, regardless of whether it was in seeds.
The most sensitive enzyme assays use lasers focused through a microscope to observe changes in single enzyme molecules as they catalyse their reactions.
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The latter were assayed based on ACC (details in Table 1), preliminarily the most sensitive RT enzyme (its standard curve was then used to estimate quantities in other enzymes).
For the latter application, it is advantageous to use the most sensitive RT enzyme available [20].
The enzyme most sensitive to cellobiose inhibition appeared to be TaCel7A, followed by AtCel7A, TrCel7A and CtCel7A (Table 1).
The Ki(Glc) values for BGs at different temperatures are listed in Table 4; the enzyme most sensitive to glucose inhibition was AtBG3, followed by TaBG3 and N188BG.
The most efficient cellobiose hydrolysis was achieved with TaBG3, followed by AtBG3 and N188BG, whereas the enzyme most sensitive to glucose inhibition was AtBG3, followed by TaBG3 and N188BG.
TaBG3 had the highest specificity constant (kcat/ KM CB) = 1.25 x 10 M-1 s-1) but was the enzyme most sensitive to glucose inhibition (Ki(Glc) = 1.14 mM).
The enzymes most sensitive to cellobiose inhibition were GH7 CBHs, followed by GH6 CBHs and EGs.
The enzymes most sensitive to cellobiose inhibition were glycoside hydrolase (GH) family 7 cellobiohydrolases (CBHs), followed by family 6 CBHs and endoglucanases (EGs).
Our hypothesis is that a mild inhibition of the V-ATPase will reveal the most sensitive biological functions for that enzyme, which will be indicated by genes that first increase transcription in response to the inhibition.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com