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The underlying reason for the reduction in PDT toxicity with low glucose concentrations cannot be attributed to changes in the intracellular concentration of PpIX, which has been previously demonstrated to influence PDT sensitivity (Wyld et al, 1997), as levels were lowest in the most sensitive cells, i.e. those treated under normal glucose concentrations.
As previously found, sheep red blood cells were the most sensitive cells [2].
In the most sensitive cells, BT474, inhibition of autophagy did not affect the outcome of TAIII treatment, indicating that autophagy is either not induced or is not protective in these cells.
We have observed that U-87 MG cells, which are used as an in vitro model of human glioblastoma are among the most sensitive cells to DT385 (Table 1).
It may be related, at least in part, to varying AA uptake between cell lines since Raji cells, which are the most sensitive cells to AA treatment, also express the highest level of sodium-dependent vitamin C transporter 2 (SVCT2) mRNA (data not shown).
In the testes of treated animals, the most sensitive cells were the premeiotic and meiotic spermatocytes.
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Additionally, PIM kinases expression levels did not predict the sensitivity of DLBCL cell lines to small-molecule inhibitors and the most sensitive cell lines expressed low levels of the kinases.
The most sensitive cell line was human ovarian cancer.
Seven compounds were further examined against the most sensitive cell lines, leukemia HL-60 TB) HL-60 TB-4.
HeLa was found to be the most sensitive cell line towards the treatment, followed by PC3, and HCT-15.
Compounds 8c and 11h were identified as the most potent ones, while HL-60, HCT116 and MDA-MB-231 were the most sensitive cell lines.
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