Additionally, PIM kinases expression levels did not predict the sensitivity of DLBCL cell lines to small-molecule inhibitors and the most sensitive cell lines expressed low levels of the kinases.
The most sensitive cell line was human ovarian cancer.
Seven compounds were further examined against the most sensitive cell lines, leukemia HL-60 TB) HL-60 TB-4.
HeLa was found to be the most sensitive cell line towards the treatment, followed by PC3, and HCT-15.
Compounds 8c and 11h were identified as the most potent ones, while HL-60, HCT116 and MDA-MB-231 were the most sensitive cell lines.
Later on, the most active methanolic extract was selected for further purification and the most sensitive cell line to the extract, HepG2, was selected for further screening (Supplementary Figure S1A).
All tested V compounds exert antiproliferative activity on MM cells in a dose dependent manner (IC50 ranges from 2.4 μM up to 14 μM) being A375 the most sensitive cell line.
For example, we observed IC50 values ranging from 26 to 890 μM for a series of triazole-linked alkyl β-D-xylopyranosides in several mammalian cell lines, with the Jurkat cell line being the most sensitive cell line [9].
The most sensitive cell line was BT-474 (IC50 = 0.21 µM), and the least sensitive one was HL-60 (IC50 = 82 µM).
One of the most sensitive cell lines, melanoma derived SK-Mel-5, over-expresses the iron efflux transporter ferroportin and has decreased expression of proteins involved in iron uptake, suggesting that it actively suppresses cytoplasmic iron.
Due to the high influence of the most sensitive cell line, MOLP-2, and the most resistant cell line, RPMI-8226 LR5, a direct comparison of these two cell lines was made.
