Overall, the data suggest that AC fixation is unacceptable for preservation of most samples, whereas FA and PFA fixation should be chosen according to the tissues and proteins to be studied.
The C-3 epimer of 25OHD3 and 1α25(OH)2D2 were quantified in most samples, whereas the 1α25(OH)2D2 and 3-epi-25OHD2 were only present in one sample (410), which contained a significant amount of endogenous 25OHD2.
Western blot analysis of normal and tumoural mammary glands from six female dogs (two in metoestrus, two in anoestrus and two after prolonged treatment with progestins) showed that PRA was either equimolar or predominant in most samples, whereas predominance of PRB was recorded in only one case [ 4].
None of the samples robustly inhibited VSV-G dependent entry, whereas most samples markedly reduced entry-driVSV-G dependentas entryted (7 ).
Several genes (PLL3, 4, 6, 7, 12, and 24) were transcriptionally silenced across most sampled tissues, whereas PLL2 was apparently expressed ubiquitously, suggesting a very general function (see Additional file 1).
In most samples (71%), only MBT was quantifiable, whereas TBTs were measured in only 14, suggesting either an old contamination or rapid degradation processes.
Substitutions of amino acids in cluster 4 (616, 631, 633, 635, 636, 636) affected most samples irrespective of peptide 601 620 inhibition, whereas those in cluster 1 (630 and 639) and cluster 2 (610, 624, 628, 629, 632, 638, 638) rarely affected antibody binding.
Second they (Safran et al, 2004) used CT-guided biopsies or fine-needle aspirates, whereas in our study most samples were true cut biopsies.
Most samples showed quite similar values (θwater ~ 29°), whereas only the sample with smaller pores showed a significantly higher θwater.
Most samples were associated with invasive infection (n = 123, 57 %), whereas the remaining samples were recovered from throat (n = 57, 27 %) and skin infections (n = 34, 16% ).
Most samples revealed positive results using a total amount of 100 ng of DNA whereas some required an increase of DNA to 500 ng per reaction.
