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As most samples stained for TLR4 and MyD88 showed similar colour intensity, from moderate to strong, no evaluation of colour intensity was performed in this study.
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While most samples revealing high mRNA levels displayed strong ATM protein staining, interestingly, some samples stained strongly despite expressing low mRNA levels; thus, there was a lack of statistical correlation between ATM mRNA and IHC-staining levels (P > 0.5).
All tissue samples stained anti-tPA positive.
Unstained samples or samples stained only with annexin V-FITC or propidium iodide were analyzed as controls.
In most samples, the staining was observed in a high percentage of cells, in more than 50% cells in all cases except two and more than 80% cells in 16 cases.
b) Sample stained with CD49D.
Supplementary Figure 5 High-resolution electron micrograph of a sample stained by NATIVE.
Sample stained with hematoxylin-eosin solution.
While the staining intensity of amylase bands in a few samples showed no increase by reduction with DTT, increased staining was seen in most samples.
Most samples from colon adenocarcinoma, breast adenocarcinoma, ovary adenocarcinoma, and lung cancer showed intermediate to strong cytoplasmic SPP1 staining in tumor cells, but the staining in normal tissues was much weaker.
Staining our synaptoneurosomes for the mitochondrial marker VDAC1 revealed the presence of synaptic mitochondria in all samples, with similar levels in most samples (Fig. 7f).
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