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For most samples, only glutamine but no glutamate was detected.
Although most samples only experience one freeze/thaw cycle, reanalysis, or inadvertent thawing during transport may occur.
In most samples only DNA with a high molecular weight was present and DNA was hardly degraded.
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Most sampled only a few apes, who were usually in captivity or in ape sanctuaries close to humans.
In most samples (71%), only MBT was quantifiable, whereas TBTs were measured in only 14, suggesting either an old contamination or rapid degradation processes.
SSAP method produces many polymorphic fragments and allows differentiation of most samples using only a single combination of specific primers [ 23, 28– 30].
All the assayed SNPs amplified in most samples and only 12 were monomorphic in all the accessions genotyped, giving a validation ratio of 92%.
Most samples exhibited only IgM or very low IgG levels, suggesting acute-phase disease or the beginning of class switching (data not shown).
Studies of artificially mixed M. tuberculosis strains before and after culture showed that culturing can reduce the clonal complexity of the strains and that, in most samples (6/10), only 1 strain will be identified in mixed infections after culture (3 ).
The caveat to integrating such a broad range of environmental studies is that, for most metagenomic samples, only a few physico-chemical parameters are available (usually temperature and pH).
We did not model random effects on plot level to account for local, hence unstructured spatial effects because for most sample plots only one height was measured (Table 1).
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