For most samples, we perform multiple stages of laser annealing and characterization, with laser power increased between each stage.
Many segmentation algorithms are designed to analyse samples individually (see, e.g., [ 3- 16] and references therein), while most studies involve multiple samples, multiple tracks, or both.
Somatic mutation analyses of the immunoglobulin heavy gene locus by ultra-deep sequencing in chronic lymphocytic leukaemia detected multiple subclones in most samples, which supports this model (Campbell et al, 2008).
In addition, comparison of multiple samples requires analyzing most samples in TOF mode only, saving considerable machine-time usage.
As most samples are wildtype for any given amplicon, multiple samples will make it easy to distinguish a mutation from the wildtype variation.
The forest floor was collected in multiple layers at most sampling dates, usually the Oie (the litter and fermented layers) and Oa (humic layers).
Collection was carried out by EM at mobile Red Cross vans at 10 locations (Bellbowrie, Capalaba, Dayboro, Eagle Farm, Kenmore, Mount Ommaney, South Brisbane, St Lucia, Virginia, and Woolloongabba) distributed across the city, most with multiple sampling days and 5 10 samples per day, between March and June , 2009
For most patients, multiple samples were obtained at sequential times covering the acute, convalescent, and recovered phase of the disease.
The samples were collected May 2003 July 2005 at different locations, most with multiple sampling sites.
While most genotypes were detected only once, five (7%) were sampled multiple times.
