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All 3 Robo proteins were localized in the GE at this stage (Supplementary Fig. 1 D – F ). Specifically, Robo1 and Robo3 showed similar patterns of expression both within the MGE and LGE, and overlapped in regions where Robo1 protein was most robustly localized.
The protocol with 3 cycles, a block duration (dp) of 15 s and a total scan time (dt) of 105 s most robustly localized S2 (contralateral: lr = 71.4%, r = 0.65, dS = 1.01%; ipsilateral: lr = 100%, r = 0.6, dS = 1.14%) whereas the most time-efficient protocol to localize SI (sbc = 5, dp = 6 s, dt = 66 s) provided no robust localization of S2.
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After wild-type Lm infection of macrophages, PKCε-YFP robustly localized to membranes associated with the bacteria (Fig. 1B).
Testing the role of ExonC in mitochondrial targeting of Spire1C, we found that a GFP-tagged ExonC fusion protein robustly localized to mitochondria in expressing cells.
MitoCas9 robustly localized to mitochondria; together with gRNA targeting of mtDNA, specific cleavage of mtDNA was observed, demonstrating its functional application for mtDNA editing.
Unfortunately, due to high variability in clathrin staining on the spindle, we were unable to quantify this staining, and therefore, we generated a stable cell line expressing GFP-clathrin light chain (CLC), which robustly localized to spindles (Fig. 4b).
In agreement with a correlation between telomere processing and HOT1 telomere association, HOT1 robustly localized to telomeres on all spreads analysed with on average 87% of chromosome ends being HOT1 positive.
Gli3 was diffusely expressed in uterus and placenta, especially in ectoplacental cone (EPC) at E8.0, however, it was robustly localized in both the junctional zone and labyrinthine zone at E9.5 and E11.5.
The results show that OFA obtains robustness with extremely low performance cost, so as to reduce the requirement on the average degree from 25to1010 for robustly localizing a network.
We found that NCX1 robustly localizes to buoyant caveolin-enriched microdomains prepared from rat ventricular myocytes using a standard discontinuous sucrose gradient (Fig. 3 E ).
White matter and grey matter alterations were most robustly co-localized in the right anterior temporal lobe including the most consistently involved white matter tract, the uncinate fasciculus.
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