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Mutant osmr3, the most robust mutant, was converted in its markerless derivative (osmr3f).
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We found that K125E mutant cells have the most robust proliferation (9), fastest clonogenic formation ability (9) and the lowest p53 transcriptional activity (as presented by the luciferase assay and BAX mRNA levels; Fig. 2).
Mechanistically, most of them elevate the Aβ42/Aβ40 ratio (Table 2), with the most robust data being obtained for the V717 FAD mutants [ 45- 47].
From the 12,480 individual Db10-derived clones that were tested, 12 mutants (0.1% of all the mutants screened) were selected as showing the most robust reduction in virulence.
While the wild-type protein showed the most robust interaction with LC3B, the interaction of the K7A/D69A double mutant (Lamark et al., 2003) was reduced but still readily detectable.
We focused on the H4 RH17,18CY mutant for subsequent studies as it caused the most robust suppression.
Hence, we chose the 90 most robust RNAi clones that ablated intestinal gcs-1p::gfp expression in prdx-2 mutants in each of four separate screenings (see Additional file 1: Table S3).
BOTTOM LINE The most robust perks.
Which topology is the most robust?
It also features the most robust API options.
Even the most robust cybersecurity tech isn't 100% effective.
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