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Of the three transmembrane ephrinB ligands (B1, B2, B3), ephrinB2 has the most robust interaction with TBC1d24 in Co-IP analyses (Fig. 1a).
The most robust interaction of TDP-43 with p65 in both coimmunoprecipitation experiments was detected in brain extracts from individuals with MCI (Subjects 8 and 15).
While the wild-type protein showed the most robust interaction with LC3B, the interaction of the K7A/D69A double mutant (Lamark et al., 2003) was reduced but still readily detectable.
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As said earlier, carboxyl and pyridyl functional groups are known to form the most robust intermolecular interactions.
"And I asked him whether he wanted to know what the most robust gene-environment interaction was with the narrowest windows of action and the shortest time to onset of a significant effect.
20 Thus, genes from the monoamine neurotransmission system are among the most thoroughly studied in psychiatric genetics and in particular in the aetiology of mood disorders, and have provided perhaps the most robust evidence so far for interaction with various types of risk environments, including childhood environment.
The most robust network consists of 1246 interactions among 461 genes.
FRET imaged by FLIM is one of the most robust techniques to detect molecular interactions with comparatively high spatial resolution [10], [11] making this the method of choice to quantify [Hb] in live cells.
Although interaction between the patient and the practitioner is suggested as the most robust component of context effect, behaviour, communication and interaction between patient and practitioner are difficult to change and may be hard to reproduce.
Since protein-protein interactions are very well studied as compared to those of ncRNAs, by analyzing both protein-coding and ncRNAs together, the most robust networks resulting from this analysis would only contain protein-protein interactions, essentially losing the weaker, less-studied ncRNA interactions.
No statistical interaction was observed within this analysis (interaction p value=0.108), hence indicating that the overall analysis provided the most robust estimate of effect.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com