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The American first amendment is probably the most robust expression and enshrinement of the primacy of free speech in an open society.
When we immunolabeled for PLCβ1 4, we found the most robust expression for PLCβ3 in primary HBE cells (data not shown).
34 Given the high biological heterogeneity of GC, this integrative process allowed us to define a small set of genes with the most robust expression signatures that correlate with GC tumorigenesis.
Even though our RFP transgenic ES cell lines failed to maintain strong uniform expression with in vitro passage, we selected three lines exhibiting the most robust expression for making chimeras, in order to ascertain whether we could achieve, or possibly recover, RFP expression in animals.
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Among the lines generated, Tau4RTg2652 had the most robust tau expression levels and stable reproducible expression of early tauopathy markers relative to non-Tg mice.
This strategy aimed at selecting the most robust gene expression changes present through multiple time-points and helped us to efficiently eliminate the "zigzagging" expression profiles especially observed with genes expressed at low level (raw expression <100, see methods).
In contrast, in cerebellar granule cells and in differentiated SH-SY5Y neuroblastoma cultures, the CMV promoter directed the most robust transgene expression.
The finding that the hippocampus rather than the mPFC showed the most robust IEG expression changes with Nestlet treatment is consistent with the finding that nest building appears to reflect brain hippocampus function [24].
Consequently, we have adopted RT-PCR, the most robust gene expression method available for gene discovery studies.
In order to identify the most robust gene expression signatures among SSc esophageal biopsies in the absence of potentially confounding and overlapping pathologies, we analyzed SSc samples alone.
Among the non-E7 polymers, 536 60 (w/w) and 536 90 (w/w) nanoparticles had the most robust gene expression (respectively, 59 ± 5% and 65±2%2% in 9L cells, and 73±± 3% and 71 ±5%% in F98 cells).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com