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We find that most ribosomal binding of SUTs exhibits a strikingly different pattern from binding to coding sequences.
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Ribosomal protection proteins are believed to bind to the ribosome, allosterically decreasing the affinity of tetracycline for its ribosomal binding site and allowing protein synthesis to occur.
Where multiple start codons or ORFs were indicated, the presence of a potential ribosomal binding site (RBS) was used to help identify the most likely ORF.
DNA was synthesized to encode either protein using codons most frequently used in streptococci; each coding region was preceded by an engineered ribosomal binding site and restriction sites for cloning a promoter.
ribosomal binding site.
Each araC-pBAD promoter precedes a ribosomal binding site and a multicloning site (MCS).
A ribosomal binding site in the mRNA (bold print) is located upstream of the initiation codon (ATG, position 1).
Localized random mutagenesis was performed on the crtW gene including the upstream ribosomal binding site (RBS).
The expression of GPPS was optimized by using ribosomal binding sites (RBSs) designed to have different translation initiation rates (TIRs).
Through these primers, appropriate linker sequences and a ribosomal binding site (RBS) sequence were attached to each gene or operon to facilitate the Gibson assembly [44].
To enhance GABA production in C. glutamicum, ribosomal binding site (RBS) sequence and promoter were searched and optimized for increasing the expression efficiency of gadB2.
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