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Among them, the secretory pathway was one of the most represented pathways.
The most represented pathways were those related to cell proliferation, migration, and VSMC activation.
The most represented pathways were 'metabolism' (3887 unigenes, 48.18 %) (Fig. 3).
Another group of disease-associated pathways were those related to cancer, which ranked second among the most represented pathways.
Among the most represented pathways were also purine and pyrimidine metabolism, methane metabolism, and phenylpropanoid biosynthesis (Additional file 9).
Among the most represented pathways are metabolic pathways, which are important for parasite survival inside the host.
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KEGG pathway analyses indicated that ion transport is the most represented pathway corresponding to DETs upregulated in male pupal heads.
KEGG pathway analyses indicated that proteolysis is the most represented pathway corresponding to DETs upregulated in the female pupal head.
The five most represented pathway subcategories were: translation; energy metabolism; folding, sorting and degradation; carbohydrate metabolism; and amino acid metabolism.
In addition, the target enrichment test of the miRNAs using metabolic pathways demonstrated that "protein modification" was the most represented pathway followed by "carbohydrate degradation," "glycan metabolism," "biosynthesis," and "phospholipid metabolism".
Our analysis showed the three most significantly represented pathways to be acute phase response, followed by actin cytoskeleton signaling and tight junction signaling (Figure 2).
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