Whenever multiple transcript variants exist, the most represented and/or the longest isoform was chosen.
Proteins involved in development processes were the second most represented (20%) and comprise Leonardo 14-3-3 14-3-3 14-3-33 protein epsilon (14-3-3 epsilon), actin-87E isoform 2 (Ptx1), cathepsin D (cathd), imaginal disc growth factor 4 (idgf4) and lethal (2)37 (l(2)37cc).
Mouse and human isoforms were compared in greater detail as they are the most represented in the current databases.
Presumably, these represent isoforms of the same protein that retain their immunogenicity.
Metalloproteinases, major constituents of spider venoms [ 15], were the most highly represented grouping with 45 different isoforms identified.
In the naïve cortex, tau protein was detected by immunoblots as a cluster of bands approx. 52 68 kDa, most likely representing the various isoforms and phosphorylation states of tau protein.
Western blot analysis and densitometry showed that of the three Skp1 isoforms, Gn-Skp1 was most heavily represented in peak fraction 10, whereas HO-Skp1 was most heavily represented in fraction 11 and unmodified Skp1 in fraction 12. Thus, Gn-Skp1 preferentially associated in this competitive setting in which the Fbs1 concentration was limiting.
Within each protein type, the isoform induced in SMARC1N-PN1 was encoded by the most highly represented contig in the BAT93 EST collection.
At some stages it is even possible to see two bands right on top of each other (e.g. columella at breaker stage), which most likely represent two different ACO isoforms.
As a result, a shortest path (minimum weight path) represents the most possible isoform of the gene.
