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A unique sequence similar to the hydrophobin II from H. jecorina was the most represented gene.
The most represented gene ontology terms among deregulated transcripts in esl1 Δ esl2 Δ mutants are in the classes of transport, carbohydrate metabolism, and one-carbon metabolism.
'transcription factor activity'nucleosomeome' and 'programmed cell death' were among the most represented gene ontology (GO) categories (Supporting Information Fig S10, microarray analysis- GEO Dataset GSE21237).
TuSp1 was surprisingly abundant, ranking 10th most represented gene of the ones that are more abundant in MA glands than cephalothoraxes, directly following MaSp2.
The budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1) was the most represented gene being present in 13 of the 20 gene sets.
In addition, the analysis of the ten most represented gene groups, based on the most expressed GO Slim for plants [ 31], confirmed the homogeneity of gene expression in the buds of the different species studied (Table 2).
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Among the most represented genes are representatives of sugar-digesting enzymes and members of an apparently Scolytid-specific gene family of unknown function.
Notably, among the most represented genes of this class were those encoding the different subunits (CoxL, CoxM, CoxS) of carbon monoxide dehydrogenase (CODH) (Table 3).
These strict parameters were established to limit the search only to the most represented genes.
To identify the participants in PWD response, the most represented genes in each sample were identified and the number of up and down regulated genes were analysed.
The two most represented genes in the M library were Cytochrome C oxidase subunit 3 (EC 1.9.3.1) and Bos taurus hypothetical protein, LOC783526, with 13 and 12 ESTs respectively.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com