Histologically, EOC is thought to originate from the single layer of ovarian surface epithelium (OSE) [5], [7], [20] [22], which therefore should be the most representative control tissue.
The most representative tumor tissue block was chosen from each case and 5 μm sections were taken to poly-L lysin coated slides for immunuhistochemical staining.
The present study may therefore be most representative of postmitotic tissues or may highlight features characteristic of the nervous system.
We explored the possibility of only using several most representative biomarkers for tissue differentiation.
The ETP peptide is localized close to adipocytes within the breast tumour, which are the most representative cytotypes in breast tissues and they secrete considerable amount of collagen VI.
Serial paraffin sections (5 μm thick) were cut from the blocks with the most representative amount of tumour tissue, as determined in haematoxylin and eosin-stained slides.
The most representative block of tumor tissue was chosen in each case, and 5-μm sections were obtained and mounted on poly-L-lysine-coated slides for immunohistochemical staining.
After review of H&E-stained sections, three 1 mm cores were identified from the most representative areas of the tumour tissue, then re-embedded into recipient TMA blocks using an MTA-1 Manual Tissue Microarrayer (Beecher Instruments, Sun Prairie, WI, USA).
The most representative tumor and normal peritumoral tissue sections were immunostained using the Benchmark XT automatic stainer (Ventana Medical Systems, SA Stasbourg, France).
Two core tissues containing the most representative tumor areas (3 mm or 5 mm in diameter) were taken from the individual donor blocks and arranged into new recipient TMA paraffin blocks using a trephine apparatus.
