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Each case was represented by 2 tissue cores, 1.5 mm in diameter, obtained from the most representative areas of primary invasive tumors or in some cases (9.6%) from synchronous axillary lymph node metastases and re-embedded in 51 microarray blocks.
Continuous gas monitoring of CO2 in soils [36] carried out at different areas of the Vulcano island shows that the mean values of CO2 degassing in the most representative areas were lower than those of the previous year, in agreement with the observation carried out in the area of Vulcano Porto during the monthly surveys [33].
Duplicate 0.6 mm core biopsies from the most representative areas of tumor cells (neoplastic epithelial cells) and tumor stroma were collected from each surgical specimen using a tissue-arraying instrument (Beecher Instruments, Silver Springs, MD).
All sarcomas were histologically reviewed by two trained pathologists (S.S. and A.V). and the most representative areas of viable tumor cells (neoplastic cells) were carefully selected and marked on the hematoxylin and eosin (H&E -stained slides and sampled for tH&E -stainedcroarray blockslidess).
According to ethical guidelines, Tissue Micro-Array (TMA) was built using the most representative areas from each single case.
Tissue Microarray (TMA) was built using the most representative areas from each single case with one replicate.
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The studies led to the selection of the most representative area within the Douro Demarcated Region of about 10% of the total area later entitled Alto Douro Wine Region.
The most representative area was chosen for subsequent metric analysis.
17 In brief, the most representative area of DCIS was marked on the original hematoxylin and eosin (H&E) stained section.
The most representative area of the tumours was punched to produce the TMA blocks including four cores (diameter 0.6 mm) from each of the original blocks.
The most representative area of the tumour was punched to produce a hereditary breast cancer tissue microarray including two cores (diameter 0.6 mm) from all of the original blocks.
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