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First, taking advantage of the high variability of gene expression levels across the adult human brain, we were able to identify the most reliable microarray probe for each gene and remove poorly behaving probes.
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To solve these problems, and in order to identify the most reliable oligos for a definitive turbot microarray, a pilot microarray was developed.
Foreground intensities were background-corrected using the normexp approach (maximum likelihood variant "mle", offset = 50) as previously reported as the most reliable method for two-colour microarrays where background estimates are available (Ritchie et al. [ 29]; Silver et al. [ 30]).
In this report, we compared RNAs isolated from macrodissected, of which only surrounding healthy tissue had been removed, and microdissected rectal carcinoma samples by microarray analysis in order to determine the most reliable approach to detect the expression of tumor cell-derived genes by microarray analysis.
At first glance, a general evaluation indicates that the Gasch et al. microarray data is the second most reliable source for functional data (Fig. 5a).
The most reliable reference genes were validated by the comparison of qRT-PCR and microarray data Fig. 2 WRS of the reference gene candidates.
Robust microarray averaging (RMA) analysis of array data provided the most reliable comparisons, especially for weakly expressed genes.
In this study, we determined the most reliable way to detect the expression of tumor cell-derived genes by microarray analysis: macrodissection or microdissection.
Most reliable?
He is the most reliable.
Perhaps not the most reliable sources.
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CEO of Professional Science Editing for Scientists @ prosciediting.com