SIMCOMP was used to define the most relevant substrates and products of each reaction.
Metabolites involved in metabolic pathways that have already been inferred may also help in selecting the most relevant substrates.
As mentioned earlier, and in part because of the systematic difficulty of measuring PKA activity in vivo, an important supplement is to measure the net effect of PKA activity targeted towards the most relevant substrates (possibly reflecting local PKA activities) by measuring Gli processing.
As suggested by the reviewers, we have now measured the effects of PKA on its most relevant substrates in Hh signaling – Gli2 and Gli3, and have added these new data to the revised manuscript. 1) PKA is known to inhibit Gli2 translocation to the cilia tip, a process required for Gli2 activation (Tuson et al., 2011).
Subsequent studies showed that the two most relevant infectious substrates in California forests are provided by bay laurel (Umbellularia californica) and tanoak leaves, while oaks and tanoak boles appear to be non-infectious (Reviewed in Rizzo and Garbelotto 2003).
An important caveat to this approach is that the available gene expression data may come from tissues that are not necessarily the most relevant or complete substrate for the trait under consideration.
More elaborate analyses of myofibril formation within the most relevant physiological range of substrate elasticities between 15 and 90 kPa revealed that neither the number of costameres per cell, nor the mean area per α-actinin cluster or the fraction of the cell area labeled by α-actinin was significantly affected by substrate elasticity (Fig. 3).
Considering that the biogas production rate was the technical parameter that displayed the most relevant differences between the analyzed substrate options (0.99 1.45 m3 biogas m3 d−1).
TiN coatings produced by the unbalanced magnetron sputtering (UMS) technique were used to study the relationship between the most relevant deposition parameters, such as substrate bias and working gas pressure, and the residual stress level.
Although most of the relevant substrates whose dephosphorylation drives the execution of mitotic exit remain unknown, persistent Cdk activity prevents mitotic exit and return to interphase in all eukaryotes studied [ 3,44 46].
