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However, in this study we are aiming to combine data from several registers thereby obtaining information on most relevant confounders.
The HG diagnosis was based on Fairweather's diagnostic criteria, and information on most relevant confounders was available.
The most relevant confounders we identified were year of birth, distance to the reference health facility, and educational attainment of the father.
eGFR and phosphate were included in the second model, as they were judged the most relevant confounders based on biological plausibility as both eGFR and phosphate potentially affects FGF23 and mortality risk but are not in the causal pathway.
Indeed, careful examination of these trials shows that they share the following common features: inclusion of the most severe patients (ARDS only, excluding acute lung injury (ALI) non-ARDS patients), and control for the most relevant confounders - that is, proning duration (usually >17 hours/day) and use of lung-protective ventilation [ 10, 16, 17].
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To find the most parsimonious and explanatory model, relevant confounders were controlled using a forward stepwise procedure by adding variables with a plausible relationship to the dependent outcomes and significant at the p < 0.2 level in bivariate analysis.
5 6 In four studies, the role of a putative protopathic bias could not be ruled out because an insufficient duration of follow-up 5 7; a lack of statistical power in subgroup analyses 8; and no consideration of the most relevant time window for exposure and ascertainment of confounders.
The five most relevant checklist items regarding selection bias, blinding and determination of confounders were used to assess risk of bias in the included studies.
Post hoc analysis revealed that adjustment for maternal intelligence accounted for most of this effect where full adjustment for a range of relevant confounders yielded a small (0.52) and non-significant effect size (95% CI −0.19 to 1.23).
In our data set, we had 1 year of daily data, which is less than that in the studies described above; the factor analysis chose only some factors, and in the model we adjusted for all potential confounders that have been shown to be the most relevant.
After adjustment for potential confounders, a clearer association emerged with the second trimester being the most relevant window of exposure.
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