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One explanation for this finding might be that monocytes are not the most relevant cells for unravelling links between genome variation and the risk factors investigated.
Since macrophages are among the most relevant cells involved in HIV-1 and T. cruzi infection, the analysis of the steps affected by co-infection was conducted on MDM.
Because breast cancer cell lines usually display very low or no expression of endogenous aromatase [ 28], the most relevant cells in which to study the response to AIs are cells transfected with the human aromatase gene.
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Similarly, lack of access to the most relevant cell types (biopsies cannot be performed arbitrarily on patients) can be a limiting factor for the method.
For the present study, we selected as the most relevant cell types: B spermatogonia (BS; premeiotic cells), pachytene spermatocytes (PS; primary spermatocytes in meiotic prophase), and round spermatids (RS; postmeiotic haploid cells).
For this purpose we used CHO-K1 and HEK293 cells - the most relevant cell lines for protein production.
Although currently HPTCs appear to be the most relevant cell type, primary cells are suboptimal, and this issue as well as the cell sourcing problem must be addressed.
The NB4 cells appear therefore to be the most relevant cell line to study cAMP effects on anthracycline-induced acute myeloid leukemic blast death.
In this study, we have analysed the impact on OS cell biology of two of the most relevant cell cycle-related, druggable kinases: AURK-A and AURK-B.
The primary significance of hEPI-NCSC is that they are biologically the most relevant cell type to generate Schwann cells, as they are direct descendants of the embryonic neural crest, which is the source of Schwann cells in the body.
Therefore, the most relevant cell viability data were obtained when this endpoint was measured in each test run, and directly within the sample used for measuring the specific endpoint (e.g., migration).
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