However, links with avian immunity for most differentially regulated transcripts remained rather hypothetical, as a large set of differentially expressed transcripts lacked functional annotation.
Included are the 14 most strongly regulated transcripts (as identified by sign test and F-test), as well as several transcripts which have a potential function during structural modifications or in ion- and acid/base regulation.
Notably, the glucose-6-phosphate/phosphate transporter 2 (GPT2) was the most strongly regulated transcript in CaLam symptomatic, infected leaves.
Also the data for the third most differentially regulated transcript in larvae from the CDH and MDH exposure groups, the aryl hydrocarbon receptor repressor (ahrr), points in the same direction.
Although most of the regulated transcripts showed only moderate changes, prealbumin, GNAL and beta-neoendorphin were highly up-regulated at 20 min (more the 10-fold).
Most of the regulated transcripts were present either in the comparisons of both genotypes in the 300 μM iron regime or when comparing B73 grown at 10 μM to 300 μM iron.
Among the 61 most over-expressed transcripts and the 10 most down-regulated transcripts, enrichments of factors involved in metabolic or signalling pathways that have not been linked to immune responses previously, such as cytoskeleton regulation, calcium signalling pathway, MAPK signalling pathway, aimnocyl-tRNA biosynthesis, and methionine, glutamate, and aminosugar metabolism, were detected.
For example, whereas UniTagSTCa-6374, annotothe to the putative extensin (Q9FSY9_CICAR), was one of the most down-regulated transcripts, UniTag STCa-19021 representing another extensin isoform (O65760_CICAR) was the most Up-regulated tag in our data set.
In particular, Fos-like antigen 2 (fosl2) and activating transcription factor 3 (atf3), which are key components of the transcription factor AP-1, were some of the most up-regulated transcripts across MR,HR and LG treatments, by 4.6-6.4 4.6-6.4-5.4 fold respectively.
