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However, the most recent speciation event took place around 4.7 million years ago splitting the two monophyletic lineages within C. neoformans var.
From this dataset, we chose of species that were fully resolved as sister species in the original analysis [69] in order to capture the most recent speciation events.
Out-duplications, in contrast, occur earlier than the most recent speciation event and produce descendants in two (or more) species.
With a second group of genes that have undergone GC since their most recent speciation event, we may have the beginnings of a pattern.
At the time of the most recent speciation event, s n −1, we have n lineages with probability 1, which is the initial value of the recursion.
As can be seen in Figure 4 (grey line), the most recent speciation event is the divergence between P. ramorum and P. sojae.
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The most recent (30 24 mya) speciation rate increase coincided with the evolution of C3 grasses, the opening up of savannah habitats23, the late Oligocene warming, and the parallel speciation of several groups of ruminants24, rodents25 and other mammal clades that may have increased the availability of host species (Fig. 1).
Additionally, Eytan et al. [41] showed very high levels of genetic differentiation between deep (>70 m) and shallow populations in the coral genus Oculina – but concluded that this most likely represents recent speciation.
It is possible that fragmentation featured more prominently in the Plurinerves during the most recent and extensive speciation event.
Although other, competing definitions of species exist [ 24, 25], most recent studies of speciation claim to have been elaborated and tested using the biological species concept [ 23, 24].
For example, V. harveyi, V. campbellii and V. rotiferianus, which form the most recent subclade of speciation within the Harveyi clade (Pascual et al., 2009), have nearly indistinguishable phenotypes (Bryant et al., 1986; Gomez-Gil et al., 2004).
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