BH01250 is the 6th most reactive antigen, and BH13260 is the 23rd most reactive antigen of the infected cat group.
BH12700 (VceA) is the 5th most reactive antigen of the infected cat group and had a mean signal intensity of 25k.
TOA25R antigen was the most reactive antigen tested, giving the highest titer in 9 of the 11 (81.8%) positive samples, consistent with a major role of this isolate as an infectious agent.
As expected, CBU_1910 was the most reactive antigen with high specificity [ 69].
The other 92 were equally reactive, including the two most reactive antigens (BH13530 (MopA) and BH07870 (LemA)).
The PGL-I and LID-1 antigens have been among the most reactive antigens tested in different populations, including multiple sites in Brazil, the Philippines, Japan, Nepal, Venezuela, Bangladesh, and India, suggesting utility in a global context [ 10, 12, 13].
The most differentially reactive antigens on the immunostrips were able to accurately predict 98% of infected cats using 3 antigens (MopA, VceA, and LemA).
The two most differentially reactive antigens are equally reactive in both types I and II, and subunit vaccines including these antigens should be ideal candidates for protection against both types I and II of B. henselae.
The three most differentially reactive antigens in the protein microararay BH13530, BH07870, BH12700 (p-value = 7.0×10−14, 2.3×10−13, and 3.3×10−8, respectively) were also the most significantly different in the immunostrip, showing validation of the microarray and transference to a separate diagnostic platform (p-value = 1.39×10−14, 1.73×10−13, and 4.0×10−10 respectively).
The same was true for the second most type-specific differentially reactive antigen (BH01250).
Considering the low immunogenicity and the consequent difficulties in obtaining efficient antibodies targeting small carbohydrate antigens, we based our strategy on Tn-antigen clusterization using different polymeric scaffolds and on a stepwise selection of the most reactive antibody producing clones.
