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About the mechanism of enhanced radiosensitivity, Turner et al. reported metallic materials may arrest cells at the G2/M phase, the most radiosensitive phase of the cell cycle [33].
Zerumbone treatment alone (and in combination with radiation) arrested cells in G2/M (Fig. 2C), the most radiosensitive phase of the cell cycle.
Paclitaxel is a microtubule-stabilising agent that blocks the cell cycle in the G2 and M phase, the most radiosensitive phase.
Cell cycle analysis showed that FGFR inhibition leads to a decrease of cells in the G2/M phase, being the most radiosensitive phase of the cell cycle [ 32], but also a decrease in radioresistant S phase cells.
It is well known that PTX enhances the radiosensitivity of tumour cells by inducing cell cycle arrest at the G2/M phase, the most radiosensitive phase of the cell cycle (Terasima and Tolmach, 1963; Sinclair and Morton, 1966).
Taking into account that the S phase is not reported to be the most radiosensitive phase of the cell cycle (Sinclair, 1972; Tang et al, 1997), the question remains whether the influence on the cell cycle indeed plays a role in radiosensitisation.
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The developing brains of mice prenatally exposed to °Co γ-irradiation were examined at the most radiosensitive stage with immunohistochemistry using an anti-MK antibody (Sun et al., 1997).
Docetaxel has also demonstrated activity as a radio sensitizer in a number of preclinical and clinical studies by blocking the cell cycle in the most radiosensitive G2/M phase.
Sorafenib (Sb) is a multiple kinase inhibitor targeting both tumour cell proliferation and angiogenesis that may further act as a potent radiosensitizer by arresting cells in the most radiosensitive cell cycle phase.
Therefore, exposure to MF for longer times or the use of higher concentrations of cisplatin could sensitize HeLa cell line by inducing G2/M arrest, the most radiosensitive cell cycle phase [ 49, 50].
It is possible that either of these antihormonal treatments sensitizes to HeLa cell line by inducing G2/M arrest, the most radiosensitive cell cycle phase, and by enhancing the capacity of cisplatin to induce apoptosis.
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