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However, the inherent limitation of kinetic studies using cell populations [1], [3], [8], [9] is that transient activities that are not synchronized from cell to cell cannot be detected even by the most quantitative assays (Fig. 1A) [11], [11].
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To have the most effective quantitative assay of virus infectivity and to compare the infectious titer with quantitative molecular assays, we optimized a plaque assay (determining the PFU/mL).
Not only has this greatly impeded broad adoption of absolute quantification, the complexities of implementing even the most basic quantitative assay hinders access to real-time qPCR technologies, particularly for casual users.
From an application perspective, assessing biological significance within the context of absolute number of target molecules can enhance the utility of most, if not all, quantitative assays.
Ideally, we would use the most comprehensive and quantitative assay available, WGBS.
Our most recent research activity devoted to quantitative assays, obtained through the detection of single molecule binding events was published in PNAS.
Here, we used the recently described MT-PCR method [ 11] to develop quantitative assays for the most important causes of ILI and the identification of Influenza A subtypes, and evaluate their performance in two clinical laboratories.
The AHL detection bioassays are most commonly performed by overlay method while quantitative assays are performed by liquid cultures.
Across all three quantitative assays, the strains with the most visually striking phenotypes (UWOPS87-242.1 for settling, 322134S for invasion, and SK1 and 322134S for adhesion) all correspond to the highest recorded quantitative measurements.
Evaluation of quantitative assays was restricted to dilutions of 0.0 log10 to −2.5 log10, a range over which the assays of most participants produced comparable data.
Podinovskaia, M. et al. Dynamic quantitative assays of phagosomal function.
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