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Most published SSRs are located in the intergenic regions [ 4].
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An alignment was also made with the published SSRs (Table 2).
All of the 500 SSRs, together with 95 previously published SSRs, were used to detect polymorphism among six F1 progenies and their parents.
The normally segregating markers, which comprised 268 newly developed SSRs, 37 previously published SSRs and 53 sequence-related amplified polymorphism markers, were used for genetic map construction.
The forward primers of published SSRs were labelled at their 5'-end with the fluorescent dye HEX Applied Biosystemss).
The amplification of published SSRs and sequences harbouring SNPs by uniplex and multiplex PCR was performed under identical reaction conditions.
Although we also examined several published SSR markers in IRGSP (2005), they were not applicable to the linkage analysis.
Recently, 23 SSR loci were identified in M. graminicola, 21 of which could be positioned on the existing linkage map along with two previously published SSR loci [21], [22].
using a set of previously published SSR markers is described.
Using the published SSR-primers we found the same fragment lengths as reported in literature.
In cassava, the number of published SSR markers is limited compared to other major crops.
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