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Specifically, the most proximal locus (Adh) exhibits higher sequence diversity in populations with a high frequency of fused X chromosomes than in populations with a low frequency of fused X chromosomes, whereas the most distal locus (tim) exhibits the inverse relationship.
Inverse PCR and DNA sequencing were performed to identify the most proximal locus and determine the EP orientation relative to the coding region.
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CRISPRs were assigned to the most proximal cas locus, in a few cases the repeat detected by CRISPRfinder was changed manually to match homologous CRISPR repeats.
These loci were mapped to their most proximal gene expressed in either mouse or human (lines) and cumulative fraction is shown (y-axis).
This is a significant improvement over the previous strategies [ 18, 24, 29], which only considered the most proximal and distal PA sites annotated for individual gene loci.
It is probably better to draw a more anatomically correct mushroom body schematics (with a bifurcation), with Glu, ACh, and GABA mapped to the correct anatomical loci (right now Glu is the most proximal to the cell body, which is misleading as in reality it is the most distal at the tip of medial lobes).
This analysis adopts a simplifying conservative assumption that lincRNA loci are more likely to regulate transcription of the most proximal gene than of other nearby genes.
To test whether this effect is a simple consequence of our locus definition, in which intergenic space is split in half, we repeated this experiment using an alternative locus definition, in which a non-coding element is associated with the gene that has the most proximal transcription start site to the element.
A proximal reference wire was fixed and tensioned to the most proximal ring.
Removal of some of the most proximal pins (those fixing the proximal osteotomy) once it shows signs of satisfactory healing.
The most proximal intervention to prevent painkiller (opioid) overdose deaths is to administer, either intravenously or intranasal, naloxone, an FDA-approved medication with well-established efficacy and safety.
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CEO of Professional Science Editing for Scientists @ prosciediting.com