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Although most primary human cells lack telomerase, the enzyme is upregulated in more than 90% of invasive breast cancers.
Consequently, for most primary human cell types such data remain unavailable.
Most primary human NF1+/- cells are a challenge to obtain, however lymphoblastoid cell lines (LCLs) have been collected from large NF1 kindreds.
This can, most likely, be attributed to the fact that 50% of all cancer cell lines and most primary human cancers are TRAIL resistant.
Soluble TRAIL variants showed no cytotoxic effects on most primary human cells and were well tolerated in mice and chimpanzees [ 4, 5].
Like in the skin-melanoma nest model, the pro-apoptotic effect of TRAIL+cisplatin was tumor selective in the skin-melanoma spheroid model, as most primary human fibroblasts and keratinocytes survived (zoomed Figure 6f).
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Glioblastoma multiforme (GBM) is the most common primary human malignant brain tumour and is among the most lethal of all cancers.
In most normal primary human cells, γH2AX foci are relatively rare so that DSBs can be detected by nonlethal radiation doses in the mGy range [ 5].
Water Humans can survive for more than three weeks without food, but water is the most primary element to human survival, second only to air.
Therefore, we next asked whether the hit compounds have similar effects on additional β cell lines and, most importantly, on primary human β cells.
The vectors using the MSCV LTR, the GALV LTR, and the PGK promoter were the most efficient at transducing primary human CD34+ cord blood progenitors under the conditions employed.
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