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Isolates were selected to represent diverse sources and to include the six most prevalent sequence types (ST) and most frequent alleles for each locus.
The approach was investigated using 47 C. jejuni isolates, previously characterized by classical MLST, representing isolates from diverse environmental, animal and clinical sources and including the six most prevalent sequence types (ST) and the most frequent alleles.
The most prevalent sequence type was the mosaic PBP 2 X (86.6 %, 129/149).
The 115-bp sense-fragment is completely included within P16 exon-1, the most prevalent sequence investigated for P16 methylation.
ST-21 is one of the most prevalent sequence types of C. jejuni present in a broad range of hosts [ 14, 15, 17- 19, 46].
It is noteworthy that the most prevalent sequence UpsB-Q-1 (dCAGGGTTAAGGGTATAACTTTAGGGGTTAGGGTT) adopts a single structure which is stable in physiological conditions (37°C and 150 mM K+).
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The most prevalent sequences are in color.
While the most common number of genes per domain sequence was one, 190 domain sequences were found in at least two genes and the most prevalent domain sequence (DFRPTAPGHSPGVGH) was identified in 53 different genes across the rosid clade of eudicots.
Three deleterious BRCA germline mutations have been observed in about 15% families and in about 3% non-familial breast cancer patients from North Sardinia (BRCA2 mutations were the most prevalent BRCA sequence variations and a single variant, BRCA2-8765delAG, was the most recurrent mutation with a founder effect in our population) [ 23- 25].
The relevance of LINE-1 methylation quantification as a marker of global DNA methylation is supported by the fact that LINE-1 is the most prevalent repetitive sequence in the human genome (approximately 17% of the genome) [ 20] and that about one-third of DNA methylation in the genome occurs in these elements [ 35].
The most prevalent read sequences for the putative mature microRNA and star sequence from one representative sample are boxed in red and purple, respectively.
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