The most predominant functional categories are cell cycle, cell division, cell proliferation, response to stress, immune response and cell adhesion (see Supplementary Table 4 in Additional file 2), consistent with known pathological mechanisms of cancer.
Among innate immune cells, neutrophils are the most rapid and predominant cell type transmigrating into CF airways, while in adaptive immunity, T-helper cell type 2 (Th2) and Th17 cell responses are predominant [16], while regulatory T cell responses are impaired [18].
For detailed characterization of the cell types, we focused on the largest aggregation present in the segment A1. Primarily based on the expression of peroxidasin-GFP (pxn- GFP) and NimC1/P1, we found the cluster to house a large number of plasmatocytes, the most predominant differentiated blood cell type (Jung et al., 2005).
Among the CD45+ cells, the most predominant were macrophages, followed by T cells, mast cells, and B cells [ 32].
ΔNp63α is the longest and is the most predominant isotype expressed in squamous cell carcinoma (SCC) cells [ 3- 5].
PRMT1 is the most predominant methyltransferase in mammalian cells [18] and is responsible for the majority of arginine methylation, thus the vast majority of research has been focused on this enzyme [19].
Myofibroblasts, defined as fibroblasts with α-smooth muscle actin (SMA) expression [ 5], constitute the most predominant carcinoma-associated stromal cells, and have been found to be involved in the production of different proteases responsible for invasion [ 6- 8], as well as in the stimulation of the proliferation of cancer cells [ 9, 10].
Cell adhesion-cell migration was among the most predominant GO categories in each tissue type, indicating a conserved role for Twist1 in cell adhesion-migration in multiple cellular contexts.
