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The most potent fraction was subjected to bioassay guided fractionation with column chromatography.
As in the ERα yeast screen, S-CMAL was the most potent fraction of CMAL.
MCF-7 cells were treated with the most potent fraction of each sample for 24 and 48 h.
Nonetheless, the most potent fraction appears to be n-butanol fractions for all the three plants evaluated.
MCF-7 cells were treated with the most potent fraction (those with the lowest IC50) and pure compounds for 8, 16, 24 and 45 h.
Finally, the most potent fraction i.e. ethyl acetate was selected for the determination of possible anti-inflammatory mechanism using various inflammatory phlogistic agents in mice.
Similar(46)
The most potent fractions were chosen for further identification using the chromatographic and spectroscopic methods as follows.
When using the PubChem database instead of the NIST library (in step 6) to search for compounds matching the molecular formulas determined in the three selected, most potent fractions (RP3NP2, RP3NP5, and RP3NP6), hardly any significant new results were obtained.
The chemical formulas determined in the three most potent fractions (RP3NP2, RP3NP5, and RP3NP6) were searched in PubChem, and results were compared with those obtained searching the NIST library.
The apoptotic effect of the most potent fractions F1 and F2 of DCOE on MDA-MB-231 cells was determined by Annexin V-FITC staining assay and measured by C6 flow cytometer (BD Accuri Cytometers, Ann Arbor, MI USA).
Because CSP-AU1 was one of the most potent fractions for activation of TNF, IL-6, and GM-CSF production, we next determined whether this sub-fraction could modulate production of other cytokines by PBMCs using a multi-cytokine ELISA semiquantitative array.
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