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There was extensive GFAP positivity with most positive cells showing morphologic features of gliofibrillary oligodendrocytes.
Interestingly, although some basal cells were undergoing DNA replication, most positive cells were found in suprabasal layers (Fig. 5d g).
Multiple bright foci were rarely observed in a single cell; most positive cells had a single bright focus.
Although some basal cells expressed cyclin A, most positive cells were found within the first suprabasal layers (peribasal), coexpressed with the postmitotic terminal differentiation keratins K1 and K10 (Fig. 4b,d and Fig. S1c e).
It should be noted that for the majority of these sorting experiments, a fluorescence gate separating cells as Alexa 700 negative and positive was set such that 80% of most negative and 80% of most positive cells were sorted.
Scattered GFAP-positive cells were seen in both components, although in the GGLF they appeared consistent with tumoral astrocytes (Fig. 6h), while in the oligodendroglioma portion most positive cells looked like reactive astrocytes (Fig. 6g).
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In both control and VT2OcKO mice, a majority of mitotic cells stained positively for BrdU, and therefore most HH3PS28 positive cells indeed corresponded to cells that progressed through S phase and entered mitosis after irradiation.
Agrin and α-dystroglycan were redistributed in both neurons and astrocytes, although most positive staining cells appeared to be neurons.
By day 30 most double positive cells do not exhibit organized sarcomeres (Christoforou et al., 2013).
Most UNC5H positive cells also expressed DCC, although DCC and UNC5H were occasionally not co-expressed.
As shown in Fig. 6a, although most UTF1 positive cells are positive to both GFRα1 and PLZF, a subpopulation of UTF1+/GFRα1+/PLZF− SSCs is present.
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