As illustrated Fig. 9, we identified most polymorphic positions in the coding sequence (18 out of a total of 29 which are reported) among which 9 SNPs induce amino acid change.
In the PR, the most polymorphic positions were 14, 40, 65, 68 and 70.
Genotyping of 861 SNP positions in the three wild rats resulted in 438 polymorphic positions, whereas the most polymorphic combination of inbred strains in this experiment (BN, BH, and SHR) yielded 427 SNPs.
Researchers can choose the most polymorphic candidate SSRs according to position and personal preference.
For the class II DRB locus (the most polymorphic class II gene) primers at positions 61 79 and 707 727 generated a 667 bp amplicon that is universally conserved, except for auxiliary DRB3 alleles, which contain a single nucleotide mismatch in the 5' primer (C74G).
These positions are located within the most polymorphic region; more specific rather than degenerated primers are needed at these sites.
Polymorphic position is indicated by vertical line.
ISMAP02 locus 1 was observed at polymorphic position in MAP S5 compared to MAP K10.
The most polymorphic autosomal locus was D12S391, which presented 38 different sequence-based alleles.
Human leukocyte antigen (HLA) genes are the most polymorphic loci in the human genome and encode the highly polymorphic molecules critically involved in immune responses.
