Finally, using as criterion for defining the 5' end of a transcript the location of a SL addition site, most of the polycistronic transcripts obtained after the initial assembling could be split up, giving a total number of 10 285 transcripts (Table 1).
miRNAs are frequently clustered in the genome (miRBase) and are most likely expressed from polycistronic transcripts.
According to our data, there were at least 15 polycistronic transcripts in FD transcriptome, most of them involving ribosomal proteins.
Read-mapping and coverage statistics also gave insights into Polytomella mitochondrial transcriptional architecture, revealing polycistronic transcripts and the expression of telomeres and palindromic genes.
A portion of these polycistronic transcripts may reflect only low-level polycistronic transcription, rather than polycistronic transcripts serving as the dominant isoforms but the rate of polycistronic transcription cannot be readily determined with available data.
Most protein-coding genes of cp genome are transcribed as polycistronic transcripts and transcriptome analysis is to be designed in particular to avoid cross-contamination between the nuclear poyA-containing and organellar polyA-lacking transcripts.
In T. cruzi, most genes are transcribed by RNA Polymerase II which generates polycistronic transcripts in a run-through fashion.
Most of the mtDNA genes are transcribed as almost genome-length polycistronic transcripts (see Box 2 ) that are next processed to produce individual mRNA and tRNA molecules.
Most of miRNAs are clustered and shared similar expression patterns, implying that they are transcribed as polycistronic transcripts [ 40, 41].
