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The most overrepresented terms in both networks included various types of kinase activity, stress responses, immune responses and processes related to cell death.
The unigenes were finally classified into 11 categories based on molecular function, and the two most overrepresented terms were binding (nucleotide binding, protein binding, chromatin binding) and catalysis (see Additional file 2).
The most overrepresented terms among species-specific alleles were related to oxidation-reduction processes (66 of 707 genes with this term) and heme binding proteins (18 of 707 genes).
To further decrease the number of candidate terms, the 25 most overrepresented terms from the development set compared to the population set were used to develop the free-text part of the search strategies in PubMed/Medline.
Both SLX4 and HRQ1 were enriched for processes related to DNA metabolism and repair, and postreplication repair was one of the most overrepresented terms (5.45- and 6.42-fold enrichment, respectively).
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The most overrepresented term in the set (Additional file 2: Table S1) was 'regulation of gene expression' (q < 2.7e-31) wasch wassociatedted with over a third (36.7 %) of the genes tested.
GO analysis of significantly up-regulated genes in phosphate-limited cultures show microautophagy (P < 1 × 10-10) and phosphate-related terms (phosphate transport; P < 1 × 10-6) as the most overrepresented GO terms.
The most overrepresented GO terms are listed in Tables 1, 2, 3, 4, 5, 6. Full listings of overrepresented GO terms are provided in Tables S1, S2, S3, S4, and listings of the significantly changed genes associated with these GO terms are provided in Data Sets S1, S2, S3.
We also discovered suites of related genes that would not be obvious a priori candidates for soil adaptation: one of the most overrepresented GO terms is microtubule-based movement (p<0.001).
The two most overrepresented GO terms were structural constituent of ribosome and structural molecule activity (Additional file 3).
The detailed information of top five most overrepresented GO terms of mouse bivalent genes is shown in Supplementary Table S2.
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