The most overrepresented pathway corresponding to the 730 AWM-predicted target genes of the three TF was HIF-1 (Additional file 5: Figure S1).
To identify the influence of each gene on the integrin signaling pathway, which was the most overrepresented pathway, a gene plot was produced using the gene set determine from the global test.
Analysis of the same gene group for Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways produced the Jak-STAT pathway as the second-most overrepresented pathway (Table S3), with many other genes being clearly linked to pathways relevant for innate immunity.
Table 1 shows ten of the most significantly overrepresented pathways (see complete list in Supplemental Table 2, inside Additional File 1).
In addition, alanine and aspartate metabolism were the second and third most significantly overrepresented pathways (P < 0.003), respectively, represented by DLAT (dihydrolipoamide S-acetyltransferase) and NARS (asparaginyl-tRNA synthetase).
Indeed, four regulated genes, including the insulin receptor itself (insra, ppp1r3b, eife1a, cblb), mapped onto the KEGG Insulin Signalling Pathwayy.' The most overrepresented KEGG pathway was otherwise that for 'Pyrimidine metabolism' (9 genes, P = 0.0013).
In particular, transcripts belonging to the PKA signaling pathway, such as PDE3B, PDE4A and PDE4D phosphodiesterases, turned out to be the most overrepresented genes in such list, with differential expression patterns, which were confirmed also in the additional set of paired diagnosis-relapse BCR ABL1-positive ALL samples analyzed with Human Exon 1.0 ST array.
Indeed the most overrepresented demographic group in the military - African-American women - is the same group least likely to support the war in Iraq.
African-Americans and Hispanics are the most overrepresented groups; a study of death-row exonerations found that wrongfully convicted black defendants outnumbered whites by six to one.
