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Finally the most optimal threshold producing the nearest size and number of residential areas was chosen (Table 1).
We found 150 mg/L to be the most optimal threshold to dichotomise serum CRP levels.
Secondly, the reader chose the most optimal threshold separating the dense tissue from the non-dense tissue.
With the iQ Multiplex Powermix the amplification curves for all targets had relatively small exponential phases, resulting in a small range for determining the most optimal threshold.
Addition of CRP as a dichotomised variable, where 30 mg/L was the most optimal threshold, resulted in an area under the curve of 0.77 (0.73 to 0.81) with a Hosmer-Lemeshow test of 9.67 (df=8, P=0.29).
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White bars: minimum NDC obtained using the most optimal thresholds for each event; black bars: NDC obtained using our thresholds.
In addition, the most optimal Pstart threshold value was determined.
It is important to select the most optimal Blastn e-value threshold, so that we will not miss any real homologs, and also avoid too many false positive hits.
However, for most of the cases, the optimal threshold selected by an index maximisation was concordant with the experimental results (see the supplementary Figure S2).
We used the lower cut-point of eight or above, validated in most primary care studies as the optimal threshold for identifying hazardous or harmful drinking and used in US national alcohol surveys [ 19, 20].
Analysis performed during iterations helped us to select the optimal threshold required for the most pertinent selection of differentially expressed genes.
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