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In order to arrive at the most optimal assay with respect to the signal window and minimal interference from compound autofluorescence, we compared the performance of ethidium bromide, the dye most commonly used in this assay, to ThO, in side-by-side titration experiments using an unlabeled version of the optimized APE1 substrate (Figure 1C).
The 6 s/well fractions resulted in the most optimal assay conditions in terms of the Z' factor (0.66).
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The results demonstrated that the enrichment of nonselective medium for 1 h prior to both PCR or bacterial cell culture assays yielded the most optimal condition.
Furthermore, the debate on the most accurate method to determine serum 25(OH D levels is still ongoing [ 36], but the competitive protein-binding (CPB) assay applied in this study might not be the most optimal method.
In both assays, D-penicillamine was less effective than captopril, indicating the need for further research into the most optimal free sulfhydryl group donor for clinical applications.
But she said she felt confident that she had done the most optimal planning possible.
We discuss the optimal assay development methods for several different scenarios.
Thirdly, the optimal assay volume (0.5, 1, 2, 4, 8 and 16 µl) was determined using the Streptavidin - Biotin-PE assay.
In addition, the most optimal Pstart threshold value was determined.
To determine the most optimal management, modelling is often used.
The data from this most optimal protocol are presented here.
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