Sentence examples for most off-target from inspiring English sources

Similarly, most off-target methylation decreased within 7 days post-transfection (Supplementary Fig. 5k), which is somewhat surprising given the low expression levels of TET enzymes (Supplementary Fig. 5l).

From 3 libraries we were able to collect the most off-target sensitive transcripts.

Chemical modification of the siRNA, such as inserting a 2′- O-methyl ribose in the nucleotide in the second position of the guide strand, could reduce silencing of most off-target transcripts with complementarity to the siRNA guide [ 100].

Developmental neurotoxicity is one of the most prominent off-target toxic risks of insecticides.

Of the three redox couples perturbed, glutaredoxin 1, attenuation produced the most numerous off-target effects with 10/28 genes assayed showing statistically significant changes.

Very interesting phenomenon is that both transcripts NM_001030055 and NM_001173 of gene ARHGAP5 are the most sensitive off-target transcripts in both libraries, Ambion and Dharmacon, but are targeted by different siRNAs.

Limited analysis showed that no mutations were observed at the most likely off-target sequences of the dpy-3 gene in the mutant F2 animals, suggesting that this method can induce specifically targeted mutations.

In plants, mutants disrupting the BSM gene have been shown to be embryo-lethal (Babiychuk et al., 2011), therefore we suspected that the BSM gene is the most likely off-target of mutations in the abp1-1 and abp1-1s alleles.

The Grx1-silenced cells showed the most widespread off-target changes with respect to the control cells, with mRNA levels of 8 additional proteins affected: Prx4, IDH2, Ref1, Rac2, CAT, SOD2, G6PD, Trx2.

Bioinformatic analysis was used to identify the most likely putative off-target sites and to design PCR primers that would amplify these sequences from genomic DNA of founder enhancer deletion mouse lines.

We designed a new class of α-benzylidene γ-butyrolactone inhibitors that showed low micromolar potencies against Dyrk1A and/or Clk1 and a good selectivity profile among the most frequently reported off-target kinases.