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However, according to the accessibility data of the buried residues in the membrane bilayer, most of mutants showed a slow reaction rate with 4-PDS within membrane bilayer (Figure 6B and Table 2).
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Immunoblotting analysis of the solubilized extracts of cells expressing each mutant showed that most of the mutants showed a similar level of opsin expression compared with that of the basal mutant (data not shown).
Most of the mutants still retained high activity on β-carotene, however, the F213L single mutant and the R203W/F213L double mutant that yielded the highest improvement for astaxanthin production showed decreased activity for canthaxanthin production.
Most of the mutants were found in the N6 subtype, which included six mutants (10.91%), followed by N1, with four (10.26%), and N9, with three mutants (27.27%).
Most of the mutants and transgenic plants reported with modified lignin are xylem mutants [ 24– 26].
On the other hand, a non-central node corresponds to a mutant which exhibits similar (redundant) metabolic capabilities with most of the mutants of the graph.
While 2C and most of the mutants had equal or higher affinity for the Y5 variant than for QL9, mutant CDR1beta exhibited 8-fold lower affinity for Y5 compared to QL9.
Results from these experiments show that most of the mutants display growth defects at high and low temperatures, as well as other mutant phenotypes.
Most of the mutants in the S. pombe and S. cerevisiae ORF deletion sets are null mutations, and haploid mutants lacking essential genes are not present.
Most of the mutants arrived without tidings of love, and some instead carried a software payload designed to erase victims' disks once the message's attachment was opened.
The molecular docking study presents a plausible explanation for reaction specificity alteration and hydrolysis inhibition for most of the mutants.
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