In preliminary experiments we tested inhibitors of RAS converting enzyme 1 (RCE1), and of isoprenylcysteine carboxyl methyltransferase (ICMT), but most of them induced cell death when used in commonly cited concentrations and time-scales in our model system.
Current acute myeloid leukemia (AML) disease models face severe limitations because most of them induce un-physiological gene expressions that do not represent conditions in AML patients and/or depend on external promoters for regulation of gene expression/repression.
Most of them induce cell cycle arrest at G1 phase, leading to either the inhibition of cell proliferation or induction of apoptosis in tumor cells.
They are often referred to as "dauer pheromones" because most of them induce formation of long-lived and highly stress resistant dauer larvae.
In addition, most of them induce the collapse of a pre-existing IF network such as in 3T3 cells, although several mutant desmin proteins integrate well, just like the wild-type protein, into the vimentin network [ 9, 11, 16, 167].
90% of all the pathway nodes were associated with more than one ADR, implying the disturbance of most pathways induced concurrency of multiple ADRs.
Pho4 function can be conveniently monitored by measuring the activity of the secreted acid phosphatase, Pho5, which is one of the most highly induced members of the PHO response [ 19, 20].
Similarly, the set of false positives, FP, corresponds to the fraction of P which are not in the 1% of most induced genes.
The gene XTR6 (At4g25810) was selected because it was among one of the most highly induced transcripts of any gene on our salt treated ABR17-transgenic A. thalina microarray (Table 3, Figure 4).
Interestingly, the top 50 of most highly induced genes contain a remarkably high number of established PPARα targets, regulation of which was conserved in mouse hepatocytes.
