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Most new microRNAs are under-represented in both conditions.
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Therefore, we predict that new microRNAs expressed early in human development would not likely be conserved across most species.
Kloosterman, W. P. et al. Cloning and expression of new microRNAs from zebrafish.
Most new ballparks are smaller.
So are most New Zealanders.
The significantly high proportion of new microRNAs matching seed sequences from previously known microRNAs suggests the presence of new family members, perhaps by a seed-conservative evolutionary mechanism.
We expect that many of these new microRNAs will function in neural differentiation.
Initial analysis of new microRNAs suggests that they are expressed in a more biologically-specific manner and are less conserved than previously-identified microRNAs.
Small RNAs were sequenced and those predicted microRNAs that were enriched at least 4-fold over IgG controls were selected, yielding 146 new microRNAs (Files S2 and S5).
While many previously-known microRNAs are found clustered in genome, we find that the closest distance between adjacent microRNAs within this new list of 146 is 21 kb, but four of the new microRNAs appear to cluster with previously-known microRNAs (neighbors listed in File S2).
The dataset was screened for new microRNAs as described previously (Marco et al. 2010, 2013a; Marco and Griffiths-Jones 2012), but no new microRNAs were found.
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