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When using a standard supra-threshold mechanical stimulus (ramp and hold displacement between 48 and 96 μm, for 2 s every 60 s), the mechanoreceptor response in most neurons remained very stable for periods greater than 20 min up to 90 min (data not shown).
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In parallel, we have also analyzed axon pathfinding inside rhombic lips explants by time-lapse experiments since it seemed that most of the GFP-electroporated neurons remained inside the explant from TTL−/− embryos.
Most newly produced neurons remaining in the OB after learning are still present several weeks later and can develop thousands of spines [9], [14], [15], [29].
The Map1b-positive pyramidal neurons remained normally orientated.
Excitatory neurons regained normal levels of response, while narrow-spiking (inhibitory) neurons remained less active.
After 72 h, only 20% of EGFP-positive neurons remained in pBudEGFP/APP-transfected neurons.
(K ) Laser ablation of ASN eliminated the single neuron (ASN) while other PPK neurons remained intact.
These reductions are important to consider when assessing the effect of connectivity changes on dynamics, but they illustrate that the general profile of connections between neuron groups is not substantially altered connections from P2/3 to P2/3 and P5 neurons remain the most numerous, for example.
Cajal Retzius neurons remain in the most superficial cortical layer, the marginal zone, whereas subplate neurons descend into a deeper layer below the cortical plate neurons.
All other neurons remain optically 'dark'dark
If all neurons remain below the threshold, then (I_{mathrm{syn}} equiv0).
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