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We included a mitotic shake-off to remove most mitotic cells before FACS analysis.
While in most mitotic cells, the cues that direct cell polarization are often extrinsic, coming from the environment or certain cortical landmarks [13], [14], the molecular cues for asymmetric meiotic divisions of oocytes remain poorly understood.
We have previously observed that most mitotic cells in the E18 HP express EGFR (Carrillo-García et al., 2010).
Neurobasal and Hibernate are defined media formulations optimized for enriching neuronal growth at low densities and selecting against most mitotic cells.
Most mitotic cells in both the saline- and lipopolysaccharide-treated animals went through mitosis with a cleavage plane near vertical (75 90°).
After exposure to 25 μg/ml EcEE, most mitotic cells presented abnormalities (80%and63%3% after 24 and 48 h, respectively).
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Although most mitotic intestinal cells are considered to be transit amplifying rather than stem cells [ 22], dividing cells may persist as long as their mitotic compartment or niche persists.
Most vector-transfected mitotic cells showed totally depolymerized spindle MTs or exhibited rare K-MTs (Figure 5C).
However, in most of the mitotic cells having multiple mt nucleating foci only two pericentrin positive centres were observed.
Although loss of RP is very useful marker of senescence in cell culture, this marker may not play a key role in age-related pathologies in the organism, because most post-mitotic cells should not be able to restart proliferation anyway.
The most recognisable feature of mitotic cells is the bipolar spindle, a multi-molecular machine that capture, align and separate the sister chromatid pairs.
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