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Matrix-Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) mass spectrometry (MS) has proven effective in the analysis of most lipid classes [20].
Drosophila lipogenesis occurs in the fat cells and involves most lipid classes (Arrese and Soulages 2010).
Exposure to TBT affected levels of most lipid classes, with the exception of LPC.
We observed that most lipid classes give a linear response over two orders of magnitude of sample amount (0.2 20 μL) (Fig. S2).
However, for individuals exposed to TBT H, this correlation was lost for most lipid classes, except for PC and PS (r = 0.90 and 0.95, respectively, n = 5).
Fatty acyl chains from 16:0 to 16:4 and 18 0 to 18 4 were found in most lipid classes; for triglycerides, fatty acyl rests were also found at 14 0, 17:0, and 20 0 carbon lengths.
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Sphingomyelins, phosphatidylcholines, and phosphatidylethanolamines were the most abundant lipid classes.
The identified lipids are listed in supplementary Table S1, and the most abundant lipid classes are shown in Figure 1.
To this end, a mixture containing standards representing the most abundant lipid classes was added post-extraction to blood plasma lipids extracted for different times.
This includes e.g. plasmanylethanolamine desaturase (I), the key enzyme for the synthesis of one of the most abundant lipid classes in our body, the plasmalogens.
To implement the use of ion mobility in routine MS-based lipidomics workflows, we measured the CCS of the most common lipid classes in independent laboratories.
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