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The MEGF10 GFP fusion protein under the control of the ced-1 promoter was efficiently translated and observed at the cell surface; however, in most lines, the localization of the fusion protein displayed an additional punctuated pattern (not shown).
However, in most lines the number of the double transgenic progeny was not different from what was expected from Mendelian inheritance (Table 2), indicating that the microcephaly did not cause embryonic lethality in most lines.
For most lines, the first PCR reaction was carried out using primers Tol2-F13 and Tol2-R4 (primer sequences are listed in Additional file 9: Table S1).
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Carlos Keen now has around a dozen restaurants, most lining the dusty street that borders its central playing field.
Most line the National Mall.
Actually, "what I like to do is be the guy who's in the most scenes, has the most lines, and has the best part!
And like most lines in the sand, people tend to get on one side or the other of them.
On most lines of the Dutch railway network, where a substantial amount of block-braked trains have rough wheels, the average wheel roughness dominates over the rail roughness.
Most lines in the sand come from opinions on self-expression -- which isn't a necessity.
Try to make most lines have the same number of syllables.
Based on the pedigrees, most lines were from the U.S. and China (Fig. 3d and Additional file 2: Table S1).
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