This indicates that small RNA synthesis is most likely templated using a given gene rather than a long template covering several genes.
Because the Taq and Stoffel polymerase both reached the same optimum S/N of 100, it seems most likely that template damage via heating-cooling cycles as found by Gustafson et al. [ 29] is limiting S/N in this system.
These characteristics make these types of lipids amenable to the template-based combinatorial enumeration of virtual compound libraries, using the pre-defined lists of most likely chains and templates containing the appropriate head groups.
With the low probability of seeing two failed traces from one unique clone, we conclude that these failures are most likely a result of template DNA with 5' or 3' ends that are problematic for sequencing.
The micellar structures most likely act as soft templates that assemble growing PANI chains and/or aniline molecules into particular nanostructures and/or their precursors.
Interestingly, the new population of hits identified for both platforms shows an excess of cytosine deamination related mismatches at 5'-ends (C → T for Illumina and G → A for Helicos, Additional file 1: Figure S1, S2), suggesting that these most likely consist of DNA templates with 5'-overhangs, that were lost due to the use of a seed region.
In the same vein, the Glu and Asp contamination products with the 20 AA template h most likely resulted from the Gln and Asn stocks.
Although these negative results seemed to rule out the superposition theory, the low correspondence between synthetic and recorded data has been proposed to be most likely due to a suboptimal template estimation procedure [15], [17].
Virtual compound libraries are generated by the combinatorial enumeration of most likely chains around the specific templates, with chain lengths varying from 2 to 39 containing specific number of double bonds and their geometry.
Your vendor will, most likely: offer a selection of templates which you can edit easily, allow you to upload a completed design, or allow you to design a postcard from scratch.
