K125E is almost certainly exerting its effect in at least a dual manner: as a phosphatase dead (most likely) trapping mutant (dominant negative) and by nuclear mislocalization.
Most likely therefore ESCRT mutants were never isolated as pep mutants, unlike other endo-lysosomal trafficking complexes such as HOPS, CORVET or retromer (Jones, 1977, Genetics).
Thus, if looping was due to balancing problems caused by the bel (lhx2) mutation, most likely all homozygous bel mutants regardless of how the optic nerves project (i.e., both bel fwd and bel rev, see [5]) would be expected to have had the same tendency for such looping behaviors.
The WT strain would most likely outcompete the mutant in competitive cultures sharing the same resources.
The few remaining phenotypically wildtype females will most likely mate with mutant males.
The higher levels of ssDNA generated further from the chromosome end in cdc13-1 pin1Δ mutants, in comparison with cdc13-1 exo1Δ mostnts, most likely accounts for their sustained metaphase arrest following telomere uncapping.
However, the observed effect on Na+ kinetics in the two mutants most likely reflects the direct effect of mutation on Pi binding and translocation.
Our analysis reveals that the underlying conformations induced by these AT1R mutants most likely represent principally different mechanisms of uncoupling the G protein, which for some mutants may be due to their increased ability to recruit β-arrestin2.
Under normal conditions (i.e., without β-Yariv treatment), no inhibition is observed in the single mutants, most likely due to gene redundancy, a conclusion supported by the observation that galt2galt5 double mutants show inhibition under normal conditions [ 16].
One can speculate why a LS ferric haem EPR signal is stronger in the valine mutant compared with the tyrosine mutant – most likely because tyrosine is larger allowing lower R84 occupancy.
