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However, all identified peptides contained either a lysine residue or a protein N-terminus at the most likely linkage position as determined by observed fragments.
Next, the inheritance of these cultivars over a pedigree was analysed using IBD estimates and the most likely linkage phases of alleles according to the software package FlexQTL™ [ 17].
For markers unassigned to a LG following that analysis, two-point LOD scores were inspected and in cases where the most likely linkage was with a marker known to be adjacent in the domestic sheep IMF map, the marker was assumed to be part of the same LG in bighorn sheep.
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Briefly, we first determined the most likely marker linkage phase for all genotyped animals (i.e. sires and daughters) using purpose-built software (Farnir, unpublished).
Evidence from clinical and genetic studies support an association of CD and sarcoidosis most likely due to linkage disequilibrium of HLA-DQ2 and -DR3.
Introgression of low glucosinolate and '0' erucic acid loci from Heera into Indian types of B. juncea with available markers [ 32] led to a reduction in yield of the recipient lines, most likely due to linkage drag.
In addition, Pir proteins (proteins with internal repeats; PIR-CWPs) are covalently linked to β-1,3-glucan, most likely via ester linkages between the γ-carboxyl group of glutamate residues, arising from glutamine, and glucose hydroxyl groups [ 7, 8].
Markers that were not included in the genome assembly were incorporated at the most likely position in the linkage map based on results from a two-point linkage analysis.
This is most likely due to mutation linkage, since K103N had high co-reversion frequency with other mutations such as M184V and L100I.
Consistent with this, these SNPs are thus associated with lack of control of HIV-1 viremia [29], most likely due to this linkage [70].
Most likely, therefore, each male linkage group corresponds to a separate chromosome.
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